Development of Low Gly m Bd 30K (P34) Allergen Breeding Lines Using Molecular Marker in Soybean [Glycine max (L.) Merr.]
نویسندگان
چکیده
منابع مشابه
Soybean [Glycine max (L.) Merr].
In this chapter we describe an Agrobacterium tumefaciens transformation method of soybean that utilizes mature half seeds and regeneration from the cotyledonary node region. This method results in fertile transformed soybean plants and transgenic seed in approximately 9 months. Using mature half seeds as starting material has proven to be a reliable method that does not require additional wound...
متن کاملIdentification of the glycosylation site of a major soybean allergen, Gly m Bd 30K.
A major soybean allergen, Gly m Bd 30K, is a glycoprotein. A peptide containing a sugar chain was isolated from an alpha-chymotrypic hydrolyzate of the allergen. The amino acid sequence analysis of the peptide showed that its sugar chain binds to the Asn170 residue. Furthermore, the sugar moiety of the allergen and peptide was shown to consist of mannose, N-acetylglucosamine, fucose, and xylose...
متن کاملTranslocation of Sulfate in Soybean (Glycine max L. Merr)1
MATERIALS AND METHODS Sulfate translocation in soybean (Glycine max L. Merr) was investigated. More than 90% of the sulfate entering the shoot system was recoverable in one or two developing trifoliate leaves. In young plants, the first trifoliate leaf contained between 10 to 20 times as much sulfate as the primary leaves, even though both types of leaf had similar rates of transpiration and ph...
متن کاملTranslocation of Sulfate in Soybean (Glycine max L. Merr).
Sulfate translocation in soybean (Glycine max L. Merr) was investigated. More than 90% of the sulfate entering the shoot system was recoverable in one or two developing trifoliate leaves. In young plants, the first trifoliate leaf contained between 10 to 20 times as much sulfate as the primary leaves, even though both types of leaf had similar rates of transpiration and photosynthesis. We concl...
متن کاملSymbiosis-stimulated chitinase isoenzymes of soybean (Glycine max (L.) Merr.)
and to be colonized by arbuscular mycorrhiza (AM) fungi (Harrison, 1997). Often, nodule formation by a Isoforms of endochitinase in soybean were studied in given bacterial strain is restricted to only a few host plant relation to root symbiosis. Five selected cultivars difgenera, and varieties of a given legume species may differ fering in their nodulation potential were inoculated in nodule fo...
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ژورنال
عنوان ژورنال: Plant Breeding and Biotechnology
سال: 2013
ISSN: 2287-9366
DOI: 10.9787/pbb.2013.1.3.298